In vitro androgenesis in pepper (Capsicum annuum L.) and the affecting factors on success: II. Carbohydrate source and antioxidants

Microspore cells develop into male gametophytes, which are released as pollen. Under certain stress conditions, the developmental pathway of microspores can be transformed an embryo instead pollen with haploid technology. In this experiment, 2 pepper breeding lines (G-1 and G-2) 4 nutrient media formed Murashige & Skoog (1962-MS) + 30 g L-1 sucrose or maltose without vitamins antioxidants. 0.05 mg biotin (vitamin B7) 0.5 ascorbic acid C) were studied The anthers pretreated for days at 35 ºC in dark, then they incubated a climate chamber 25 16/8 hours photoperiodic conditions. highest embryos rate, development plantlet has been obtained from medium containing maltose, Although there was no significant differences between genotypes medium- I (MS sucrose), medium-II antioxidants) medium-III maltose) terms embryogenic response, difference determined medium-IV antioxidants). number G-1 increased 2.5, 6.4, 4.5 times, compared to medium-I, medium-II, respectively.